Composite

Part:BBa_K872001:Design

Designed by: Ricardo Munguía Díaz   Group: iGEM12_Ciencias-UNAM   (2012-09-26)


CO2 biosensor


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 714
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 898
    Illegal BamHI site found at 1035
    Illegal XhoI site found at 304
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 525
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1768
    Illegal BsaI.rc site found at 1850


Design Notes

BamHI restriction site added, this is a very sensitive biosensor


Source

For module I secuence can be found in https://parts.igem.org/Part:BBa_K872000

For module II TU_Delft 2010 biobrick secuence can be found in https://parts.igem.org/Part:BBa_K398331

References